Accessory sigma factors, which reprogram RNA polymerase to transcribe specific gene sets, activate bacterial adaptive responses to noxious environments. Here we reconstruct the complete sigma factor regulatory network of the human pathogen …
Summary We also studied host responses to M. tuberculosis infection using computational approaches:
characterizing the transcriptional response in infected macrophages under various small molecule perturbations using RNA-Seq analysis; understanding the dysregulation of lipid metabolism in M. tuberculosis-infected macrophages using dynamic Bayesian model and statistical analyses of heterogeneous single-cell populations. Collaborators Rutgers University Marila Gennaro Sanjay Tyagi Eduardo Sontag (now in North Eastern) Tulane University Deepak Kaushal Knowledge Synthesis Hugh Salamon
The bacterial envelope integrates essential stress-sensing and adaptive functions; thus, envelope-preserving functions are important for survival. In Gram-negative bacteria, envelope integrity during stress is maintained by the multi-gene Psp …